In one of the first measured e-bike physical activity studies, LEVER researchers Cherry, Bassett, Fitzhugh, and Langford (UTK) assessed and compared the metabolic activity generated by riding a pedal-assist e-bike, a conventional bicycle, and walking along a fixed course with a downhill, flat, and uphill sections. They estimated energy expenditure based on heart rate and ventilation rates (VO2). They found that e-bikes provide moderate physical activity (MET>3) on flat segments and downhill segments, and vigorous physical activity (MET>6) on uphill segments. Differences between e-bikes and bicycles are most pronounced in the uphill segment. For e-bike trials, riders reported higher levels of enjoyment and lower need for a shower than in walk or conventional bicycle trials. E-bikes can contribute as an active transportation mode to meet required physical activity guidelines. This work was funded by the Southeastern Transportation Center and the Institute for a Secure and Sustainable Environment.